Friday, October 30, 2015

The question of whether iPSCs retain characteristics of their somatic origin has been a subject of considerable interest. While low-passage iPSCs may exhibit some epigenetic memory (Kim, et al., 2010), extended passaging brought the methylation patterns of iPSCs closer to ESCs (Nishino et al., 2011).

PLoS Genet. 2011 May;7(5):e1002085. doi: 10.1371/journal.pgen.1002085.

DNA methylation dynamics in human induced pluripotent stem cells over time.

Nishino K1, Toyoda M, Yamazaki-Inoue M, Fukawatase Y, Chikazawa E, Sakaguchi H, Akutsu H, Umezawa A.

Sunday, October 18, 2015

Antibody used for subcapsular implantation of HAES

Ab	Manufacturerer	Cat. No.	Web
Anti HLA　classⅠ-A, B, C, Mouse Monoclonal antibody	Hokudo co., ltd.	AB-46	http://www.hokudo.co.jp/shiyaku/reagent/HLAclassI.pdf
Human Albumin Antibody	Bethyl Laboratories, Inc.	A80-129A	https://www.bethyl.com/product/pdf/A80-129.pdf
Monoclonal Mouse Anti-Human Ki-67 Antigen Clone MIB-1	Dako	M7240	http://www.dako.com/download.pdf?objectid=104980004
Anti-MRP2 antibody [M2 Ⅲ-6]	abcam	ab3373	http://www.abcam.com/mrp2-antibody-m2-iii-6-ab3373-references.html
Cytokeratin 19 (b170)	Leica	PA0799	http://www.leicabiosystems.com/ihc-ish-fish/novocastra-reagents/primary-antibodies/details/product/cytokeratin-19/
Anti-Cytochrome P450 3A4 antibody	abcam	ab135813	http://www.abcam.co.jp/cytochrome-p450-3a4-antibody-ab135813.html

Saturday, October 17, 2015

PMD Act

Regulation to distribution by Nakayama: Is this correct?

Killer phrases and plus

Google search: Killer phrases.

Plus.

1. One subject at a time.

2. No counterproposal is yes.

3. What, By whom, By when.

TEMCELL and HS

Web site from 日刊薬業WEB

Ayumi Y

One man's Systemization of HDK1 on immortalized HFFs.

1. Banking of Ay-HDK1-MCB (10 tubes), -MWCB (x 10), and -WCB (x 10).

2. Banking of Ay-iHFF-MCB (10 tubes), -MWCB (x 10), and -WCB (x 10).

3. SOP

4. Characteristics

5. Specification

6. Record with Yoriko

HDK1 on immortalized HFFs in 3D condition.

Yugawa method.

Epidermal differentiation of XPA-, XPC- and GEN1-iPSCs.

Tuesday, October 13, 2015

Ando

ESC-derived MSC-knst

Bank of irradiated RMD9: Frozen stock 30 tubes.

iPSC: MRCiPS#25 and EdomiPS#22

No cartilage was obtained.

Manufacturing

1. knst-MCB, -WCB from research bank of pHAES-WCB

2. Propagation in Pb10.

Target disease: 1. Fabry disease, 2. Ischemic limb, 3. Ischemic heart.

Product. CellSaic: RCP and knst

PLoS Med. 2005 Jun;2(6):e161.

Derivation of multipotent mesenchymal precursors from human embryonic stem cells.

Barberi T1, Willis LM, Socci ND, Studer L.

Realization of Clinical trial or study (Mission: FIH for an endpoint of safety)
1. Product: Sheet or single forms of CellSaic with RCP and knst.
A. Starting material: Bank of pHAES-rWCB or bFF-MCB
2. POC in vivo: Ask Hiroshi Suzuki.
A. Target disease: Ischemic heart disease and Fabry heart. Ischemic limb. With FF. Like Chg-Fkwts.

Manufacturing
1. Generation of knst-rMCB
A. Generation of irradiated RMD9 feeder cells
a. Feeder cells (irrRMD9) are prepared from RMD9 as irrMEFs.
B. Cells from pHAES-rWCB is propagated on irrRMD9.

Strength
1. The CMC of the product is partly established.

Issues
1. Unclear ingredients of knst like HeartSheet.
2. knst really works like HeartSheet.
3. Competitors.
4. Milestones and personel.
5. Immunosuppressive drug.
6. Relationship of KFU and NCCHD.

knst: PSC-Stroma

Sunday, October 11, 2015

I was looking at it

I was looking at it. He was committed in the air. There was nothing wrong with it. Dive like that again and come back here in two weeks and play. Not again. Watch it.

I’m the referee on this field, not you. Stick to your job, and I will do mine. If I hear you shouting for anything again I’m gonna penalise you. This is not soccer.

Friday, October 9, 2015

PLAP-1

歯根膜細胞