1. ESC-based therapy.
2. Liver diseases: a. IEM, b. HF.
3. Academia-driven, industry-based products under PMDAct.
A. Raw-material.
B. Final product.
C. Manufacturing.
D. Sales.
Wednesday, May 20, 2015
Okzk plate
35-mm dish
200 spots/dish: diameter of the spots is 1.5 mm.
2 x 10^5/dish equal 1 x 10^7.
30 dishes make 3 x 10^8.
100-mm dish
200/month
5 x 10^7/dish
Tuesday, May 19, 2015
HAES implantation---immunohistochemistry---
K7
AE1/AE3
CD31
CD34
Vimentin
http://www.dako.com/us/ar38/p104580/prod_products.htm (specification sheet付き）
Silver
HE
-----------------------------------------------------------------------------------------------------------
-----------------------------------------------------------------------------------------------------------
腫瘍マーカー
CEA
CA125
CA19-9
c-kit
上皮系
EMA
E-cadherin
keratin
CKAE1/AE3
CK7
a-SMA
筋系
Desmin
Myoglobin
HHF35
間葉系
Vimentin
神経系
GFAP
NSE
S-100
血管、リンパ管系
CD31
CD34
白血球系
LCA
CyclinD1
内分泌系
HGM-45M1
MUC-5AC
Insulin
Glucagon
Somatostatin
hCG
肝ALB
AFP
HepPer1
増殖細胞、細胞周期
ki-67
PCNA
未分化マーカー
Oct3/4
その他
CDX2
K7 cytokeratin7 clone：OV-TL12/30、DAKO、加熱処理、50倍希釈、カタログ番号：M7018 AE1/AE3 cytokeratin clone：AE1/AE3、DAKO、加熱処理、ready to use、カタログ番号：IS053 CD31 clone：JC70A、DAKO、加熱処理、100倍希釈、カタログ番号：M0823 CD34 clone：QBEnd10、DAKO、加熱処理、100倍希釈、カタログ番号：M7165 Vimentin clone：V9、DAKO、加熱処理、200倍希釈、カタログ番号：M7020 HEP1 当施設では以下の抗体です。 Hepatocyre clone：OCH1E5、DAKO、加熱処理、200倍希釈、カタログ番号：M7158 |
ipsc ipscs ips細胞 ips
| Human induced pluripotent stem (iPS) cells | |||||||
| UtE-iPSCs : Human endometrium-derived iPS cells | |||||||
| Cell ID | Official Name | Line Name | Description | Derived cell | Vector | Reference | |
| UtE-iPS-A01 | UtE-iPS-1 | Center | UtE1104-derived iPS cells | Human endomrtrium：UtE1104 | Retrovirus | ||
| UtE-iPS-A02 | UtE-iPS-2 | Guard | UtE1104-derived iPS cells | Human endomrtrium：UtE1104 | Retrovirus | ||
| UtE-iPS-A03 | UtE-iPS-3 | Tackle | UtE1104-derived iPS cells | Human endomrtrium：UtE1104 | Retrovirus | ||
| UtE-iPS-A04 | UtE-iPS-4 | TightEnd | UtE1104-derived iPS cells | Human endomrtrium：UtE1104 | Retrovirus | ||
| UtE-iPS-A05 | UtE-iPS-5 | FullBack | UtE1104-derived iPS cells | Human endomrtrium：UtE1104 | Retrovirus | ||
| UtE-iPS-A06 | UtE-iPS-6 | TailBack | UtE1104-derived iPS cells | Human endomrtrium：UtE1104 | Retrovirus | ||
| UtE-iPS-A07 | UtE-iPS-7 | QuaterBack | UtE1104-derived iPS cells | Human endomrtrium：UtE1104 | Retrovirus | ||
| UtE-iPS-B03 | UtE-iPS-10 | Flanker | UtE1104-derived iPS cells | Human endomrtrium：UtE1104 | Retrovirus | ||
| UtE-iPS-B05 | UtE-iPS-11 | SplitEnd | UtE1104-derived iPS cells | Human endomrtrium：UtE1104 | Retrovirus | ||
| PL-iPSCs : Human placental arterial endothelium-derived iPS cells | |||||||
| Cell ID | Official Name | Line Name | Description | Derived cell | Vector | Reference | |
| PL-iPS-01 | PAE-iPS-1 | Aries | PAE551-derived iPS cells | Human placental arteriall endothelium：PL551Aｒ(PAE551) | Retrovirus | ||
| PL-iPS-02 | PAE-iPS-2 | Taurus | PAE551-derived iPS cells | Human placental arteriall endothelium：PL551Aｒ(PAE551) | Retrovirus | ||
| PL-iPS-03 | PAE-iPS-3 | Gemini | PAE551-derived iPS cells | Human placental arteriall endothelium：PL551Aｒ(PAE551) | Retrovirus | ||
| PL-iPS-04 | PAE-iPS-4 | Cancer | PAE551-derived iPS cells | Human placental arteriall endothelium：PL551Aｒ(PAE551) | Retrovirus | ||
| PL-iPS-05 | PAE-iPS-5 | Leo | PAE551-derived iPS cells | Human placental arteriall endothelium：PL551Aｒ(PAE551) | Retrovirus | ||
| PL-iPS-06 | PAE-iPS-6 | Virgo | PAE551-derived iPS cells | Human placental arteriall endothelium：PL551Aｒ(PAE551) | Retrovirus | ||
| PL-iPS-07 | PAE-iPS-7 | Libra | PAE551-derived iPS cells | Human placental arteriall endothelium：PL551Aｒ(PAE551) | Retrovirus | ||
| PL-iPS-08 | |||||||
Saturday, February 28, 2015
Citrin deficiency
http://www.ncbi.nlm.nih.gov/pubmed/20301360
Citrin deficiency can manifest in newborns as neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD), in older children as failure to thrive and dyslipidemia caused by citrin deficiency (FTTDCD), and in adults as recurrent hyperammonemia with neuropsychiatric symptoms in citrullinemia type II (CTLN2).
Friday, February 27, 2015
Critical intermediates
pHAES-WCB (Starting material) -> pHAES10 -> EB96 -> HAES
1.Propagation with MEF in KSR.
2.Period of EB formation
A.4 days in XF(-) in 96-well dishes.
3.Period of adhesion.
A.28 to 35 days in XF32.
Critical intermediate
Critical parameter
Critical procedure
pHAES-WCB (Starting material) -> pHAES10 -> EB96 -> HAES
Sampling
pHAES10: 10-cm dish cultivation just before EB formation
EB96: EBs just before passage into T25 flasks.
最終製品特性解析確認表
| 各Lot. （Lot. No.＠） | |
| 疾患マウスへの投与日 | 14/@ /@ |
| 製造日 | 14/@/@ |
| アンモニア代謝 | n=3 wells |
| 形態 | 写真 位相差 X4 (2枚）、X10 (4枚）、X20 (4枚） |
| RT-PCR | n=1, 3 wells pooled, Isongen, -80℃保存 |
| 免染 | pellet, n=1, 3 wells pooled, 4%PFA |
| 生細胞数 | トリパンブルー、カウンテス、n=3 wells |
| 工程由来不純物 | インスリン: 細胞洗浄液、-20℃、 BSA: 細胞pellet、-20℃、全wells |
| LIN28 | n=1, 3 wells pooled,RLT buffer, -80℃保存 |
| ミトコンドリア量 | 無し |
| グルタミン合成酵素 | 無し |
| フローサイトメトリー | 無し、免染で代替 |
| 院内製剤 | |
| 粒度 | カウンテス |
| 生存細胞率 | カウンテス |
| 工程由来不純物 | collagenase |
Subscribe to:
Posts (Atom)