HAES作製プロトコール

フィーダー細胞の融解・播種

pHAES-WCBの融解・播種

pHAES-WCBの拡大培養

フィーダー細胞の融解・播種

 

pHAES-WCBの継代培養

 

EB作製

pHAESの剥離・回収

 

EB作製

 

HAES作製

コラーゲンコートplate作成

 

EB回収・洗浄

EB播種

Mmb

Ymz

Ysm

Osh

Har

End

Ttm

Okr

Sjm

Msd

Mts

Sae

Ikm

Ite

Hir

Ula

Michihiro O

Takaaki Y

Ito

Kay

Sak

Kum

Collaborators

KN

KN Koichiro

1. Retina iPSCs from Peter's anomaly (PAX6 mutation) and RB.

2. 

3. ESC methylation dynamics during ESC generation from ICM cells.

4. Which vector (Sendai, retrovirus, plasmid) is the best in iPSC generation from Edom cells?

5. Murine model of liver failure

     A. Construct, 2 mo.

     B. Injection, 1 yr.

     C. SCID, cross breeding.

     D. Alb-pro---loxP---3 x pA---loxP---CAG pro---ERT2CreERT2

     E. Canine liver in mice

     F. HbT to dogs with liver failure.

     G. Murine blastocysts injected with ICM from Canine blastocysts

6. Suicidal gene into RMD9.

7. Gene therapy to cats with glutaric acid urea type II.

8. Gorila.

     

Focus

1. Novel ESC bank.

2. ESC-MSC.

3. HAES.

4. PlyD

AD project

1. Safety of the plate.

2. How to keep a secret of the manufacturing process.

3. RNA: amylase, insulin, cyp 1A2, 2B6, 3A4, albumin.

4. Fix the SOP.

5. Application; a. HAES, b. HAES organ, c. Toxicology, d. HTS.

Revenue vs OP

Operating profit 営業利益

総収入 Revenue

Plan Do See in PDCA cycle

Strategic investor vs Financial investor

Sunk (retrospective) cost vs prospective costs. 

"fixed" cost vs variable (dependent on volume) costs

China premium-----Wu

SN

Tic

Edm22#31

SEES1

Matrigel-coated 6-well plate

Feeder cells (Yub1896): XF

Medium: KSR XF

Differentiation: 15% KSR XF + AA + HRG + IGF1