http://www.ncbi.nlm.nih.gov/UniGene/library.cgi?ORG=Mm&LID=14560

NIA Mouse Mesenchymal Stem Cell cDNA Library (Long 1)

Library Description

Organism:	Mus musculus
Strain:	C3H/He mice
Developmental stage:	9-15C cells
Vector:	pCMV-SPORT6 (Invitrogen)
Vector type:	plasmid (ampicillin resistant)
Cloning site 1:	SalI
Cloning site 2:	NotI
Lab host:	DH10B

Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Dr. Akihiro Umezawa (Keio University School of Medicine, Japan). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.2 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.5 kb. The library was constructed by Yulan Piao.

Source: Dawood B. Dudekula, National Institute on Aging/National Institutes of Health

Gene Content Analysis

3,545 ESTs from this library were grouped into 1,741 UniGene entries (putative genes) [UniGene build #194, 25-Jan-2013]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTsTPM UniGene Entry

31	8745	Mm.360075	Eukaryotic translation elongation factor 1 alpha 1.
28	7898	Mm.277792	Collagen, type I, alpha 2.
27	7616	Mm.326799	Eukaryotic translation elongation factor 2.
22	6206	Mm.335315	Eukaryotic translation elongation factor 1 alpha 1.
21	5924	Mm.379939	Transcribed locus, strongly similar to NP_989488.2 EEF1A1 gene product [Gallus gallus].
20	5642	Mm.268000	Vimentin.
19	5360	Mm.87773	Heat shock protein 90, beta (Grp94), member 1.
16	4513	Mm.40828	Sequestosome 1.
15	4231	Mm.289915	Damage specific DNA binding protein 1.
14	3949	Mm.238973	ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit.
14	3949	Mm.185453	Eukaryotic translation initiation factor 4, gamma 2.
13	3667	Mm.412745	Transcribed locus, strongly similar to NP_990334.1 HSPA8 gene product [Gallus gallus].
12	3385	Mm.419997	Ubiquitin C.
12	3385	Mm.290774	Heat shock protein 8.
12	3385	Mm.282751	Anillin, actin binding protein.
12	3385	Mm.2608	Biglycan.
11	3103	Mm.290022	Eukaryotic translation initiation factor 4B.
11	3103	Mm.10299	Collagen, type V, alpha 2.
10	2821	Mm.70666	Enolase 1, alpha non-neuron.
10	2821	Mm.330160	Heat shock protein 5.
10	2821	Mm.326167	Pyruvate kinase, muscle.
10	2821	Mm.243014	Lamin A.
10	2821	Mm.182746	Myotrophin.
9	2539	Mm.485384	Nucleophosmin 1.
9	2539	Mm.34674	Myoferlin.
9	2539	Mm.277735	Collagen, type I, alpha 1.
9	2539	Mm.25610	Ubiquitin associated protein 2-like.
9	2539	Mm.248360	Annexin A1.
9	2539	Mm.243234	Proteasome (prosome, macropain) 26S subunit, non-ATPase, 2.
8	2257	Mm.88212	Tubulin, alpha 1C.
8	2257	Mm.379129	Eukaryotic translation elongation factor 1 gamma.
8	2257	Mm.291442	Secreted acidic cysteine rich glycoprotein.
8	2257	Mm.265610	Polypyrimidine tract binding protein 1.
8	2257	Mm.255066	Scaffold attachment factor B.
8	2257	Mm.247788	Chaperonin containing Tcp1, subunit 2 (beta).
8	2257	Mm.2180	Heat shock protein 90 alpha (cytosolic), class B member 1.
8	2257	Mm.123110	Spectrin beta, non-erythrocytic 1.
7	1975	Mm.46754	Solute carrier family 38, member 2.
7	1975	Mm.391967	Actin, beta.
7	1975	Mm.371545	Ribosomal protein, large, P0.
7	1975	Mm.329655	Cofilin 1, non-muscle.
7	1975	Mm.30012	High density lipoprotein (HDL) binding protein.
7	1975	Mm.289936	Phosphoserine aminotransferase 1.
7	1975	Mm.28173	Methionine-tRNA synthetase.
7	1975	Mm.27955	Eukaryotic translation initiation factor 4H.
7	1975	Mm.273538	Tubulin, beta 5 class I.
7	1975	Mm.250214	Antizyme inhibitor 1.
7	1975	Mm.240830	Disabled 2, mitogen-responsive phosphoprotein.
7	1975	Mm.212395	Retinoic acid induced 14.
7	1975	Mm.16898	3-phosphoglycerate dehydrogenase.
6	1693	Mm.428652	Septin 2.
6	1693	Mm.4237	Topoisomerase (DNA) II alpha.
6	1693	Mm.392075	Calumenin.
6	1693	Mm.378921	Gap junction protein, alpha 1.
6	1693	Mm.371570	Poly(A) binding protein, cytoplasmic 1.
6	1693	Mm.295533	Filamin, alpha.
6	1693	Mm.289662	DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked.
6	1693	Mm.28777	Plastin 3 (T-isoform).
6	1693	Mm.279923	Neural precursor cell expressed, developmentally down-regulated 4.
6	1693	Mm.27578	Melanoma antigen, family D, 1.
6	1693	Mm.27218	Mortality factor 4 like 2.
6	1693	Mm.260760	SH3-binding domain glutamic acid-rich protein like.
6	1693	Mm.255723	Muscleblind-like 1 (Drosophi

Backup

Monday, June 30, 2014

9-15c long database MSC データベース　発現 ncbi

NIA Mouse Mesenchymal Stem Cell cDNA Library (Long 1)

Library Description

Gene Content Analysis

Monday, June 30, 2014

9-15c long database MSC データベース 発現 ncbi

NIA Mouse Mesenchymal Stem Cell cDNA Library (Long 1)

Library Description

Gene Content Analysis

9-15c long database MSC データベース　発現 ncbi