http://www.ncbi.nlm.nih.gov/UniGene/library.cgi?ORG=Mm&LID=14560
NIA Mouse Mesenchymal Stem Cell cDNA Library (Long 1)
Library Description
| Organism: | Mus musculus |
| Strain: | C3H/He mice |
| Developmental stage: | 9-15C cells |
| Vector: | pCMV-SPORT6 (Invitrogen) |
| Vector type: | plasmid (ampicillin resistant) |
| Cloning site 1: | SalI |
| Cloning site 2: | NotI |
| Lab host: | DH10B |
Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Dr. Akihiro Umezawa (Keio University School of Medicine, Japan). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.2 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.5 kb. The library was constructed by Yulan Piao.
Source: Dawood B. Dudekula, National Institute on Aging/National Institutes of Health
Gene Content Analysis
3,545 ESTs from this library were grouped into 1,741 UniGene entries (putative genes) [UniGene build #194, 25-Jan-2013]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).
| 31 | 8745 |  | Mm.360075 | Eukaryotic translation elongation factor 1 alpha 1. |
| 28 | 7898 |  | Mm.277792 | Collagen, type I, alpha 2. |
| 27 | 7616 | | Mm.326799 | Eukaryotic translation elongation factor 2. |
| 22 | 6206 |  | Mm.335315 | Eukaryotic translation elongation factor 1 alpha 1. |
| 21 | 5924 | | Mm.379939 | Transcribed locus, strongly similar to NP_989488.2 EEF1A1 gene product [Gallus gallus]. |
| 20 | 5642 | | Mm.268000 | Vimentin. |
| 19 | 5360 | | Mm.87773 | Heat shock protein 90, beta (Grp94), member 1. |
| 16 | 4513 |  | Mm.40828 | Sequestosome 1. |
| 15 | 4231 | | Mm.289915 | Damage specific DNA binding protein 1. |
| 14 | 3949 | | Mm.238973 | ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit. |
| 14 | 3949 | | Mm.185453 | Eukaryotic translation initiation factor 4, gamma 2. |
| 13 | 3667 | | Mm.412745 | Transcribed locus, strongly similar to NP_990334.1 HSPA8 gene product [Gallus gallus]. |
| 12 | 3385 |  | Mm.419997 | Ubiquitin C. |
| 12 | 3385 | | Mm.290774 | Heat shock protein 8. |
| 12 | 3385 | | Mm.282751 | Anillin, actin binding protein. |
| 12 | 3385 | | Mm.2608 | Biglycan. |
| 11 | 3103 | | Mm.290022 | Eukaryotic translation initiation factor 4B. |
| 11 | 3103 | | Mm.10299 | Collagen, type V, alpha 2. |
| 10 | 2821 | | Mm.70666 | Enolase 1, alpha non-neuron. |
| 10 | 2821 | | Mm.330160 | Heat shock protein 5. |
| 10 | 2821 | | Mm.326167 | Pyruvate kinase, muscle. |
| 10 | 2821 | | Mm.243014 | Lamin A. |
| 10 | 2821 | | Mm.182746 | Myotrophin. |
| 9 | 2539 | | Mm.485384 | Nucleophosmin 1. |
| 9 | 2539 | | Mm.34674 | Myoferlin. |
| 9 | 2539 | | Mm.277735 | Collagen, type I, alpha 1. |
| 9 | 2539 | | Mm.25610 | Ubiquitin associated protein 2-like. |
| 9 | 2539 | | Mm.248360 | Annexin A1. |
| 9 | 2539 | | Mm.243234 | Proteasome (prosome, macropain) 26S subunit, non-ATPase, 2. |
| 8 | 2257 |  | Mm.88212 | Tubulin, alpha 1C. |
| 8 | 2257 | | Mm.379129 | Eukaryotic translation elongation factor 1 gamma. |
| 8 | 2257 | | Mm.291442 | Secreted acidic cysteine rich glycoprotein. |
| 8 | 2257 | | Mm.265610 | Polypyrimidine tract binding protein 1. |
| 8 | 2257 | | Mm.255066 | Scaffold attachment factor B. |
| 8 | 2257 | | Mm.247788 | Chaperonin containing Tcp1, subunit 2 (beta). |
| 8 | 2257 | | Mm.2180 | Heat shock protein 90 alpha (cytosolic), class B member 1. |
| 8 | 2257 | | Mm.123110 | Spectrin beta, non-erythrocytic 1. |
| 7 | 1975 | | Mm.46754 | Solute carrier family 38, member 2. |
| 7 | 1975 | | Mm.391967 | Actin, beta. |
| 7 | 1975 | | Mm.371545 | Ribosomal protein, large, P0. |
| 7 | 1975 | | Mm.329655 | Cofilin 1, non-muscle. |
| 7 | 1975 | | Mm.30012 | High density lipoprotein (HDL) binding protein. |
| 7 | 1975 | | Mm.289936 | Phosphoserine aminotransferase 1. |
| 7 | 1975 | | Mm.28173 | Methionine-tRNA synthetase. |
| 7 | 1975 | | Mm.27955 | Eukaryotic translation initiation factor 4H. |
| 7 | 1975 | | Mm.273538 | Tubulin, beta 5 class I. |
| 7 | 1975 | | Mm.250214 | Antizyme inhibitor 1. |
| 7 | 1975 | | Mm.240830 | Disabled 2, mitogen-responsive phosphoprotein. |
| 7 | 1975 | | Mm.212395 | Retinoic acid induced 14. |
| 7 | 1975 | | Mm.16898 | 3-phosphoglycerate dehydrogenase. |
| 6 | 1693 | | Mm.428652 | Septin 2. |
| 6 | 1693 | | Mm.4237 | Topoisomerase (DNA) II alpha. |
| 6 | 1693 | | Mm.392075 | Calumenin. |
| 6 | 1693 | | Mm.378921 | Gap junction protein, alpha 1. |
| 6 | 1693 | | Mm.371570 | Poly(A) binding protein, cytoplasmic 1. |
| 6 | 1693 | | Mm.295533 | Filamin, alpha. |
| 6 | 1693 | | Mm.289662 | DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked. |
| 6 | 1693 | | Mm.28777 | Plastin 3 (T-isoform). |
| 6 | 1693 | | Mm.279923 | Neural precursor cell expressed, developmentally down-regulated 4. |
| 6 | 1693 | | Mm.27578 | Melanoma antigen, family D, 1. |
| 6 | 1693 | | Mm.27218 | Mortality factor 4 like 2. |
| 6 | 1693 | | Mm.260760 | SH3-binding domain glutamic acid-rich protein like. |
| 6 | 1693 | | Mm.255723 | Muscleblind-like 1 (Drosophi |
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