Suggestion from Ysak.
Sunday, June 21, 2015
Saturday, June 20, 2015
YuI Yu Ikemoto
Generation of hepatocyte from GS-iPSCs.
I want hepatocytes!!
A. Four patients
B. iPSCs were successfully generated from each patient.
2. iPSC generation of immortalized hepatocytes (TKiy).
A. Two samples
a. Four lines (#2 to #5) from Hep2044 (BA).
i. Teratoma formation done (4 lines)
HE stain.
ii. Hepatocytes (#2 and #3)
the EB (iPSell w/o FGF2, 10 days, Nunclon)
and XF32 (7 days, 6-well plate) method.
b. Nine lines from Hep2013 (BA) with Xiao Yu.
i. Colony formation
ii.Freeze.
B. Future plan.
a. Exome analysis to Koj.
3. I cell iPSC with Kanz.
Monday, June 8, 2015
Sunday, June 7, 2015
ipsc ipscs ips細胞 ips
MRC5FYP
Edom22-KKN
Edom22-rRie
Edom22-SRie
UtE1104(r)KN
AM936Emi
PAE551KN
AT-iPSCsFYP
XP-iPSCsRie
XP3OS
XP40OS
XPEMB1
PetersRet2(r)KN11 lines
#5
#9
EY1422Ret(r)KN 1 line
EY1420Iris(r)Reik17 lines (until #45)
#6
#8
#37
#45
Disease (patient) iPSCs
http://blogs.yahoo.co.jp/akihiroumezawa/35410671.htmliPSCs from intact cells
Tuesday, June 2, 2015
YAndo with San
Cartilage formation by iPSCs
June 30, 2015
E8 did not work on Tik and #S31. Tip and #S31 did not proliferate, but underwent apoptosis by E8. We change the protocol to the conventional one, i.e. the conventional medium on MEF feeder cells.
Chihiro
ERCC2: repair cross-complementing rodent repair deficiency, complementation group 2 Xeroderma pigmentosum, group D
Introduction of GEN1, MAGEB10, GSTT1 and GSTM1.
Hydroxy radicals and viability after UV-irradiation.
Ayumi:
HDK1 on immortalized HFFs in 3D condition.
Yugawa method.
Epidermal differentiation of XPA-, XPC- and GEN1-iPSCs.
Plan with YS
HA to LF
|
↓
---------------------------(IND)>-------------------------------------(NDA)>------------------------
---------------------------------->-------------------------NCCDH----YS-->------------------------
M and S (Q10): YS
|
| Consignment of manufacturing|
↓
M (Q9): YS
Three way-collaboration at manufacturing
1. Place: YS ⇄ NCCHD
2. Know-how: YS ⇄ NCCHD (au)
3. Working power (human resource or assets): YS ⇄ May's
Epigenetic monitoring of PSCs
The distance between iPSCs and ESCs are larger than that between SEES and ESC-Harvard. This probably due to cultivating condition including media.
---------------------------------------------------------------
Focus on epigenome-based rgr on iPSCs/ESCs and their products
A. Q
a. Std (ESC-Harvard??)
b. Epigenetic distance from Std
c. Epigenetic quality from Std
B. Examination
a. Cobra
b. Pyrosequencing
c. Imprinting genes (H19, scrg, PEG3, MEG3)
Monday, June 1, 2015
ab antibody
K7 cytokeratin7 clone：OV-TL12/30、DAKO、加熱処理、50倍希釈、カタログ番号：M7018 AE1/AE3 cytokeratin clone：AE1/AE3、DAKO、加熱処理、ready to use、カタログ番号：IS053 CD31 clone：JC70A、DAKO、加熱処理、100倍希釈、カタログ番号：M0823 CD34 clone：QBEnd10、DAKO、加熱処理、100倍希釈、カタログ番号：M7165 Vimentin clone：V9、DAKO、加熱処理、200倍希釈、カタログ番号：M7020 HEP1 当施設では以下の抗体です。 Hepatocyre clone：OCH1E5、DAKO、加熱処理、200倍希釈、カタログ番号：M7158 |
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