YuI Yu Ikemoto

Generation of hepatocyte from GS-iPSCs.

Hh helps to orchestrate liver growth and repair. 

I want hepatocytes!!

1. Generation of Gorlin syndrome iPSC.

A. Four patients

B. iPSCs were successfully generated from each patient.
 

2. iPSC generation of immortalized hepatocytes (TKiy).

     A. Two samples

         a. Four lines (#2 to #5) from Hep2044 (BA).

            i.   Teratoma formation done (4 lines)

                HE stain.

            ii.  Hepatocytes (#2 and #3)

                the EB (iPSell w/o FGF2, 10 days, Nunclon)

and XF32 (7 days, 6-well plate) method.

         b. Nine lines from Hep2013 (BA) with Xiao Yu.

            i. Colony formation

            ii.Freeze.

B. Future plan.

a. Exome analysis to Koj.

3. I cell iPSC with Kanz.