Implantation of HAES into subcapsular region of kidney

https://docs.google.com/spreadsheets/d/17wlazq_u4LqdiHMHuFDArezPdrd80WMnn9sJHXoZbgk/edit#gid=313738524

Teeth in the tratoma by human ESCs

http://opentein.hgc.jp/browser/viewSlide.php?mode=1&sl_name=akihiroumezawa:973&annot=9

MY and PE by May

https://docs.google.com/spreadsheets/d/17wlazq_u4LqdiHMHuFDArezPdrd80WMnn9sJHXoZbgk/edit#gid=0

Extramedullary hematopoiesis in the teratoma

Extramedullary hematopoiesis in the teratoma after subcapsular injection of human ESCs.

http://opentein.hgc.jp/browser/viewSlide_bg.php?&mode=1&acc=OTi1370

村山一茂氏の論文から RSについて

科学的根拠に基づく予測・評価・判断をする。

医薬品・医療機器は法律で規制されており、「効果がある製品」であっても、法律で定められた手続きを経て承認されなければ治療に用いることはできない。

医薬品・医療機器の副作用について、前もって患者背景がわかっていれば副作用の回避や軽減も可能ではないか。有効性・安全性の最大化、副作用の最小化を図る最適化（optimization）のための考え方がRSである。

GeneExpressionデータのID

GeneExpressionデータのIDです。
まずはメールでお送りさせていただきます。

GSE42099       Gene expression signatures for human iPSC and ESC lines  Nov
05, 2015   approved  None
GSM1032378     SAMPLE 1_KhES-1_p26      Nov 05, 2015   approved  TXT
GSM1032379     SAMPLE 2_KhES-1_p26      Nov 05, 2015   approved  TXT
GSM1032380     SAMPLE 3_KhES-1_p26      Nov 05, 2015   approved  TXT
GSM1032381     SAMPLE 4_KhES-1_p26      Nov 05, 2015   approved  TXT
GSM1032382     SAMPLE 5_KhES-1_p26      Nov 05, 2015   approved  TXT
GSM1032383     SAMPLE 6_AM-iPS-3 (Sage)_p11  Nov 05, 2015   approved  TXT
GSM1032384     SAMPLE 7_AM-iPS-3 (Sage)_p12  Nov 05, 2015   approved  TXT
GSM1032385     SAMPLE 8_AM-iPS-3 (Sage)_p13  Nov 05, 2015   approved  TXT
GSM1032386     SAMPLE 9_AM-iPS-6 (Hyssop)_p7  Nov 05, 2015   approved  TXT
GSM1032387     SAMPLE 10_AM-iPS-6 (Hyssop)_p9  Nov 05, 2015   approved  TXT
GSM1032388     SAMPLE 11_AM-iPS-6 (Hyssop)_p10  Nov 05, 2015   approved  TXT
GSM1032389     SAMPLE 12_AM-iPS-8 (Thyme)_p21  Nov 05, 2015   approved  TXT
GSM1032390     SAMPLE 13_AM-iPS-8 (Thyme)_p22  Nov 05, 2015   approved  TXT
GSM1032391     SAMPLE 14_AM-iPS-8 (Thyme)_p22  Nov 05, 2015   approved  TXT
GSM1032392     SAMPLE 15_UtE-iPS-5_p23  Nov 05, 2015   approved  TXT
GSM1032393     SAMPLE 16_UtE-iPS-5_p23  Nov 05, 2015   approved  TXT
GSM1032394     SAMPLE 17_UtE-iPS-5_p23  Nov 05, 2015   approved  TXT
GSM1032395     SAMPLE 18_PAE-iPS-5_p20  Nov 05, 2015   approved  TXT
GSM1032396     SAMPLE 19_PAE-iPS-5_p20  Nov 05, 2015   approved  TXT
GSM1032397     SAMPLE 20_PAE-iPS-5_p20  Nov 05, 2015   approved  TXT
GSM1032398     SAMPLE 21_PAE-iPS-11_p23  Nov 05, 2015   approved  TXT
GSM1032399     SAMPLE 22_PAE-iPS-11_p23  Nov 05, 2015   approved  TXT
GSM1032400     SAMPLE 23_PAE-iPS-11_p23  Nov 05, 2015   approved  TXT
GSM1032401     SAMPLE 24_PAE-iPS-4_p26  Nov 05, 2015   approved  TXT
GSM1032402     SAMPLE 25_KhES-2_p31     Nov 05, 2015   approved  TXT
GSM1032403     SAMPLE 26_KhES-2_p31     Nov 05, 2015   approved  TXT
GSM1032404     SAMPLE 27_KhES-2_p32     Nov 05, 2015   approved  TXT
GSM1032405     SAMPLE 28_KhES-2_p32     Nov 05, 2015   approved  TXT
GSM1032406     SAMPLE 29_AM-iPS-2 (Clary)_p25  Nov 05, 2015   approved  TXT
GSM1032407     SAMPLE 30_AM-iPS-2 (Clary)_p26  Nov 05, 2015   approved  TXT
GSM1032408     SAMPLE 31_AM-iPS-2 (Clary)_p27  Nov 05, 2015   approved  TXT
GSM1032409     SAMPLE 32_AM-iPS-13 (Basil)_p19  Nov 05, 2015   approved  TXT
GSM1032410     SAMPLE 33_PAE-iPS-5_p25  Nov 05, 2015   approved  TXT
GSM1032411     SAMPLE 34_PAE-iPS-5_p25  Nov 05, 2015   approved  TXT
GSM1032412     SAMPLE 35_PAE-iPS-11_p28  Nov 05, 2015   approved  TXT
GSM1032413     SAMPLE 36_PAE-iPS-11_p28  Nov 05, 2015   approved  TXT
GSM1032414     SAMPLE 37_UtE-iPS-7_p26  Nov 05, 2015   approved  TXT
GSM1032415     SAMPLE 38_UtE-iPS-6_p25  Nov 05, 2015   approved  TXT
GSM1032416     SAMPLE 39_UtE-iPS-6_p25  Nov 05, 2015   approved  TXT
GSM1032417     SAMPLE 40_UtE-iPS-6_p25  Nov 05, 2015   approved  TXT
GSM1032418     SAMPLE 41_PAE-iPS-1_p26  Nov 05, 2015   approved  TXT
GSM1032419     SAMPLE 42_PAE-iPS-1_p26  Nov 05, 2015   approved  TXT
GSM1032420     SAMPLE 43_PAE-iPS-1_p26  Nov 05, 2015   approved  TXT
GSM1032421     SAMPLE 44_KhES-2_p31     Nov 05, 2015   approved  TXT
GSM1032422     SAMPLE 45_PAE-iPS-4_p26  Nov 05, 2015   approved  TXT
GSM1032423     SAMPLE 46_PAE-iPS-4_p26  Nov 05, 2015   approved  TXT
GSM1032424     SAMPLE 47_UtE-iPS-7_p26  Nov 05, 2015   approved  TXT
GSM1032425     SAMPLE 48_AM-iPS-13 (Basil)_p17  Nov 05, 2015   approved  TXT
GSM1032426     SAMPLE 49_AM-iPS-13 (Basil)_p19  Nov 05, 2015   approved  TXT
GSM1032427     SAMPLE 50_PAE-iPS-5_p25  Nov 05, 2015   approved  TXT
GSM1032428     SAMPLE 51_PAE-iPS-11_p28  Nov 05, 2015   approved  TXT
GSM1032429     SAMPLE 52_MRC-iPS-16 (Fetch)_p13  Nov 05, 2015   approved  TXT
GSM1032430     SAMPLE 53_MRC-iPS-25 (Tic)_p17  Nov 05, 2015   approved  TXT
GSM1032431     SAMPLE 54_MRC-iPS-40 (Skipper)_p14  Nov 05, 2015   approved
TXT
GSM1032432     SAMPLE 55_MRC-iPS-40 (Skipper)_p14  Nov 05, 2015   approved
TXT
GSM1032433     SAMPLE 56_MRC-iPS-40 (Skipper)_p14  Nov 05, 2015   approved
TXT
GSM1032434     SAMPLE 57_UtE-iPS-6_p30  Nov 05, 2015   approved  TXT
GSM1032435     SAMPLE 58_UtE-iPS-6_p30  Nov 05, 2015   approved  TXT
GSM1032436     SAMPLE 59_UtE-iPS-6_p30  Nov 05, 2015   approved  TXT
GSM1032437     SAMPLE 60_UtE-iPS-7_p31  Nov 05, 2015   approved  TXT
GSM1032438     SAMPLE 61_UtE-iPS-7_p31  Nov 05, 2015   approved  TXT
GSM1032439     SAMPLE 62_UtE-iPS-7_p31  Nov 05, 2015   approved  TXT
GSM1032440     SAMPLE 63_PAE-iPS-1_p31  Nov 05, 2015   approved  TXT
GSM1032441     SAMPLE 64_PAE-iPS-1_p31  Nov 05, 2015   approved  TXT
GSM1032442     SAMPLE 65_PAE-iPS-1_p31  Nov 05, 2015   approved  TXT
GSM1032443     SAMPLE 66_PAE-iPS-4_p31  Nov 05, 2015   approved  TXT
GSM1032444     SAMPLE 67_PAE-iPS-4_p31  Nov 05, 2015   approved  TXT
GSM1032445     SAMPLE 68_PAE-iPS-4_p31  Nov 05, 2015   approved  TXT
GSM1032446     SAMPLE 69_UtE-iPS-7_p26  Nov 05, 2015   approved  TXT
GSM1032447     SAMPLE 70_MRC-iPS-16 (Fetch)_p13  Nov 05, 2015   approved  TXT
GSM1032448     SAMPLE 71_MRC-iPS-16 (Fetch)_p13  Nov 05, 2015   approved  TXT
GSM1032449     SAMPLE 72_201B7_p40      Nov 05, 2015   approved  TXT
GSM1032450     SAMPLE 73_201B7_p40      Nov 05, 2015   approved  TXT
GSM1032451     SAMPLE 74_253G1_p32      Nov 05, 2015   approved  TXT
GSM1032452     SAMPLE 75_253G1_p32      Nov 05, 2015   approved  TXT
GSM1032453     SAMPLE 76_KhES-3_p28     Nov 05, 2015   approved  TXT
GSM1032454     SAMPLE 77_KhES-3_p28     Nov 05, 2015   approved  TXT
GSM1032455     SAMPLE 78_201B7_p40      Nov 05, 2015   approved  TXT
GSM1032456     SAMPLE 79_201B7_p40      Nov 05, 2015   approved  TXT
GSM1032457     SAMPLE 80_MRC-iPS-25 (Tic)_p17  Nov 05, 2015   approved  TXT
GSM1032458     SAMPLE 81_MRC-iPS-25 (Tic)_p17  Nov 05, 2015   approved  TXT
GSM1032459     SAMPLE 82_UtE-iPS-5_p18  Nov 05, 2015   approved  TXT
GSM1032460     SAMPLE 83_201B7_p53      Nov 05, 2015   approved  TXT
GSM1032461     SAMPLE 84_201B7_p53      Nov 05, 2015   approved  TXT
GSM1032462     SAMPLE 85_201B7_p53      Nov 05, 2015   approved  TXT
GSM1032463     SAMPLE 86_253G1_p42      Nov 05, 2015   approved  TXT
GSM1032464     SAMPLE 87_253G1_p42      Nov 05, 2015   approved  TXT
GSM1032465     SAMPLE 88_253G1_p42      Nov 05, 2015   approved  TXT
GSM1032466     SAMPLE 89_HUES-1_p29     Nov 05, 2015   approved  TXT
GSM1032467     SAMPLE 90_HUES-1_p29     Nov 05, 2015   approved  TXT
GSM1032468     SAMPLE 91_HUES-1_p29     Nov 05, 2015   approved  TXT
GSM1032469     SAMPLE 92_TIG/MKOS #19_p32  Nov 05, 2015   approved  TXT
GSM1032470     SAMPLE 93_TIG/MKOS #19_p32  Nov 05, 2015   approved  TXT
GSM1032471     SAMPLE 94_TIG/MKOS #19_p32  Nov 05, 2015   approved  TXT
GSM1032472     SAMPLE 95_TIG/MKOS #56_p25  Nov 05, 2015   approved  TXT
GSM1032473     SAMPLE 96_TIG/MKOS #56_p25  Nov 05, 2015   approved  TXT
GSM1032474     SAMPLE 97_TIG/MKOS #56_p25  Nov 05, 2015   approved  TXT
GSM1032475     SAMPLE 98_TIG/MKOS #106_p35  Nov 05, 2015   approved  TXT
GSM1032476     SAMPLE 99_TIG/MKOS #106_p35  Nov 05, 2015   approved  TXT
GSM1032477     SAMPLE 100_TIG/MKOS #106_p35  Nov 05, 2015   approved  TXT
GSM1032478     SAMPLE 101_TIG/MKOS #19_p37  Nov 05, 2015   approved  TXT
GSM1032479     SAMPLE 102_TIG/MKOS #19_p37  Nov 05, 2015   approved  TXT
GSM1032480     SAMPLE 103_TIG/MKOS #19_p37  Nov 05, 2015   approved  TXT
GSM1032481     SAMPLE 104_TIG/MKOS #56_p30  Nov 05, 2015   approved  TXT
GSM1032482     SAMPLE 105_253G1_p32     Nov 05, 2015   approved  TXT
GSM1032483     SAMPLE 106_253G1_p37     Nov 05, 2015   approved  TXT
GSM1032484     SAMPLE 107_253G1_p37     Nov 05, 2015   approved  TXT
GSM1032485     SAMPLE 108_253G1_p37     Nov 05, 2015   approved  TXT
GSM1032486     SAMPLE 109_201B7_p45     Nov 05, 2015   approved  TXT
GSM1032487     SAMPLE 110_201B7_p45     Nov 05, 2015   approved  TXT
GSM1032488     SAMPLE 111_201B7_p45     Nov 05, 2015   approved  TXT
GSM1032489     SAMPLE 112_KhES-3_p28    Nov 05, 2015   approved  TXT
GSM1032490     SAMPLE 113_KhES-3_p28    Nov 05, 2015   approved  TXT
GSM1032491     SAMPLE 114_KhES-3_p28    Nov 05, 2015   approved  TXT
GSM1032492     SAMPLE 115_201B7_p40     Nov 05, 2015   approved  TXT
GSM1032493     SAMPLE 116_TIG/MKOS #56_p30  Nov 05, 2015   approved  TXT

HAES dosage

1. The patient with 10% enzyme activity can survive and exhibit late-onset HA.

2. OTCD mice.

A. 20 g equals  2 x 10^6.

B. 2 kg equals 2 x 10^8.

3. in vitro activity with systematic review.

A. LB versus HAES.

B. SR of hepatocyte papers.

DOSAGE AND ADMINISTRATION

Implantation route and method

1. P3 through SMV after closure of umbilical vein.

2. UP (P3: umbilical portion of the portal vein) at neonates.

Risk management

1. Cerebral infarction due to PFO (patent foramen ovale).

2. Drug thrombus of portal vein.

3. Pulmonary infarction and embolism.

HAES dosage

Copy and paste from Saeki et al. 

Late-onset hyperammonemia was observed in a male patient with partial quantitative deficiency of ornithine carbamoyltransferase (OCT). His liver contained 10% of the normal level of OCT, but with normal kinetics. This reduced enzyme activity alone, however, could not explain the hyperammonemia. Therefore, the effects of impaired ornithine transport into mitochondria, which seemed to be a possible precipitating factor, were examined by numerical simulation of the urea cycle. The results were as follows: (a) On the assumption that the ornithine concentrations in the cytosol and mitochondrial matrix were always equal, the ammonia handling efficiency would be unchanged by decrease in the OCT concentration to 10% of normal; (b) Under conditions of impaired mitochondrial ornithine transport that had no effects on ureagenesis with a normal amount of enzyme, 10% of the normal concentration of enzyme would impair ammonia handling efficiency. Thus, impaired mitochondrial ornithine transport seemed to cause hyperammonemia in the state of partial quantitative deficiency of OCT in this case.

Registry

• Database from maternal and child health handbook and national health insurance.
• --->>> Pregnancy and drug
• 
  

HLA B2G disruption

Disruption of B2G and/or HLA class 1A/B

Financial buyer versus Strategic buyer

Financial buyer

Strategic buyer

Marina

AT

Drugs to be tested:

1. Splicing modulators.

2. Anti-oxidants

A. Vit E

B. N-acetyl-cystein

AT Treatment:

1. Bethametazon (steroid: dexamethazon) to irradiation and neural differentiation.

2. IgG

Anti-sense morpholin

1. Readthrough drug for DMD.

RALD: Ras-associated ALPS-like disorder

AAV1-locus

PiggyBac-ATM

ATM: mini-gne

electroporation

LD

Generation of iPSCs

Perspectives of Saeko with Kenta and Takaaki

Stickies for Saeko group (Saeko, Kenta and Takaaki).

1. in vivo experiments with iPSC-Hep.

2. Exome analysis to see SNP of cyp.

3. Treatment in vivo and in vitro with low molecules such as acetaminophen. 

What Saeko did in her NCCHD period. Kenta will take care of Saeko's. Takaaki Difference between Saeko method and HAES SOP.

LC-BA with intact drainage

Target disease for HAES

肝臓に関するサイト

http://ncgm-regenerative-medicine.org/research/r4.html

Organ mass of the liver---Prof Itaru Kojima

http://hepato.umin.jp/kouryu/kouryu20.html

小島教授　群馬大学

The mass of a healthy human liver is approximately 1.2–1.5 kg (2.4–3.0 lb). The liver's mass comprises one-fortieth to one-fiftieth of a total adult body's weight and one-eighteenth of the body's weight during infancy.

The liver is the only human internal organ capable of natural regeneration of lost tissue; as little as 25% of a liver can regenerate into a whole liver.

腎結石再発予防ガイドライン

Ann Intern Med, 161:659, 2014

・小まめに水分摂取。おしっこ　2L以上。

・お薬。

Nasu pptx

Chondrocyte-driven chondrogenesis in vivo---on web.

If you want to download this, click below.

https://sites.google.com/site/akihiroumezawa/storage-2/%E8%BB%9F%E9%AA%A8%E3%83%BC%E6%A2%85%E6%B2%A2%E5%85%88%E7%94%9F%E6%8F%90%E5%87%BA.pptx?attredirects=0&d=1

正解や法則が大好き

正解や法則がなくても平気。