ESC-derived MSC-knst
Bank of irradiated RMD9: Frozen stock 30 tubes.
iPSC: MRCiPS#25 and EdomiPS#22
No cartilage was obtained.
Manufacturing
1. knst-MCB, -WCB from research bank of pHAES-WCB
2. Propagation in Pb10.
Target disease: 1. Fabry disease, 2. Ischemic limb, 3. Ischemic heart.
Product. CellSaic: RCP and knst
PLoS Med. 2005 Jun;2(6):e161.
Derivation of multipotent mesenchymal precursors from human embryonic stem cells.
Realization of Clinical trial or study (Mission: FIH for an endpoint of safety)
1. Product: Sheet or single forms of CellSaic with RCP and knst.
A. Starting material: Bank of pHAES-rWCB or bFF-MCB
2. POC in vivo: Ask Hiroshi Suzuki.
A. Target disease: Ischemic heart disease and Fabry heart. Ischemic limb. With FF. Like Chg-Fkwts.
Manufacturing
1. Generation of knst-rMCB
A. Generation of irradiated RMD9 feeder cells
a. Feeder cells (irrRMD9) are prepared from RMD9 as irrMEFs.
B. Cells from pHAES-rWCB is propagated on irrRMD9.
Strength
1. The CMC of the product is partly established.
Issues
1. Unclear ingredients of knst like HeartSheet.
2. knst really works like HeartSheet.
3. Competitors.
4. Milestones and personel.
5. Immunosuppressive drug.
6. Relationship of KFU and NCCHD.
knst: PSC-Stroma
1. Product: Sheet or single forms of CellSaic with RCP and knst.
A. Starting material: Bank of pHAES-rWCB or bFF-MCB
2. POC in vivo: Ask Hiroshi Suzuki.
A. Target disease: Ischemic heart disease and Fabry heart. Ischemic limb. With FF. Like Chg-Fkwts.
Manufacturing
1. Generation of knst-rMCB
A. Generation of irradiated RMD9 feeder cells
a. Feeder cells (irrRMD9) are prepared from RMD9 as irrMEFs.
B. Cells from pHAES-rWCB is propagated on irrRMD9.
Strength
1. The CMC of the product is partly established.
Issues
1. Unclear ingredients of knst like HeartSheet.
2. knst really works like HeartSheet.
3. Competitors.
4. Milestones and personel.
5. Immunosuppressive drug.
6. Relationship of KFU and NCCHD.
knst: PSC-Stroma
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