Sunday, November 20, 2011

知らなかったこと "Transfection" and "Transduction"

学生時代を含めて全然知らなくて、間違いを指摘されたこと。反省したい。でも、他にもたくさん間違って使用しているような気がするな。どうやったら、正確な語彙を学べるのかな。

The technically distinct terms "Transfection" and"Transduction" are very loosely used throughout the manuscript.Please note that when nucleic acids are introduced to cells through viral infection, the method is referred as transduction.



Wikipedia (http://en.wikipedia.org/wiki/Cell_culture#Transfection_and_transduction)より

Transfection and transduction

Another common method for manipulating cells involves the introduction of foreign DNA by transfection. This is often performed to cause cells to express a protein of interest. More recently, the transfection of RNAi constructs have been realized as a convenient mechanism for suppressing the expression of a particular gene/protein. DNA can also be inserted into cells using viruses, in methods referred to as transduction, infection or transformation. Viruses, as parasitic agents, are well suited to introducing DNA into cells, as this is a part of their normal course of reproduction.




No comments:

Post a Comment