785 up-r at aE. Fourteen genes were selected for further cardiomyogenic analysis. PA and AM919CL was infected with 14 genes. STR analysis eliminates possibility of contamination. Karyotypes are intact. in vivo implantation analysis reveals immature c formation with transformation. Withdrawal experiments of single genes shows 9 genes alone are capable of generating colonies.
2nd: 9 genes
3rd: 3 genes
AM919CL-2nd is best for further analysis, based on RT-PCR of marker genes. 2nd#1 and 2nd#2 do not show karyotypic abnormality. in vitro differentiation of 3rd#3 was not successful.
2nd#2
Sucloning, please.
Immortalized, maybe. II down-regulation during cultivation.
Thursday, October 29, 2009
RIの教え
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