Friday, February 27, 2015

Critical intermediates

pHAES-WCB (Starting material) -> pHAES10 -> EB96 -> HAES
1.Propagation with MEF in KSR.
2.Period of EB formation
A.4 days in XF(-) in 96-well dishes.
3.Period of adhesion.
A.28 to 35 days in XF32.

Critical intermediate
Critical parameter
Critical procedure

pHAES-WCB (Starting material) -> pHAES10 -> EB96 -> HAES

Sampling
pHAES10: 10-cm dish cultivation just before EB formation
EB96: EBs just before passage into T25 flasks.


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