Determination of critical quality attributes (CQA) of the final product and critical intermediates

in Japanese

Morphology under phase contrast microscopy

Morphology by cell pellet (HE, PAS, immunocytochemistry by the yellow antibodies)

Viability by Countes after trypan blue stain.

qRT-PCR (Alb, AFP, OTC, CPS1)

Implantation into the subcapsular tissue of the kidney

Ammonia metabolism in vitro

Ammonia metabolism in vivo

Morphology by cell pellet (Oil Red O)

ICG test

qRT-PCR (Cyp 1A2, 2B6, 3A4)

Genomics

Will not do:

Flow cytometry