Michihiro: XPC(Hacchan)-iPSCs with Epidermal differentiation

XP3KA-iPSC#106, #138

1. Deposition of XP3KA-iPSC#100, #106, #125, #135, #138 to NIBIO

2. Neural differentiation: Alive.

3. Stress H202 to the medium of the neurally differentiated XP3KA-iPSC

MO project

Hayakawa's XPC iPSCs (XP3KA parental cells): XP3KA-iPSC.

A. Five lines (XP3KA-iPSC#100, #106, #125, #135, #138) from XP3KA parental cells.

            a.   Exome analysis to Koj: not done

            b.   Epidermal cell differentiation: not done.

         c.   Frozen stocks done.

 d.   Sendai virus

i. #100: No sendai virus.

ii. the others have sendai virus.

e.   Undifferentiated state

i. Alp

ii. Teratoma formation: not done.

Control: Edom-iPSCs.

Graduation thesis:

Validation of iPSC undifferentiated state.

Purpose:

Nucleotide alteration rate of XPC-iPSCs after UV-irradiation with viability test.

Cosmetic purpose.