Difference between the Saeko method (S) and HAES SOP (H)

1.Period of EB formation

A. S: 10 days in KSR in 96-well dishes in iPSellon without bFGF.

B.H:　3 days (4 to 7 days) in XF(-) in 10-cell dishes or 96-well dishes.

2.Period of adhesion.

A. S:    7 days in XF32 .

B.H:   a month in XF32.

XF(-): XF32 without the growth factors.