Hiroka

gRNAs 1-4, 2-4, 3-4, and 4-1were successful on 293 cells. 

Neural stem cells from EdomiPS#S31 cells were transfected with the 1-4, 2-4, 3-4, and 4-1 (ATM disrupting gRNA) the Crisper-Cas9 system. Transfection efficiencies were quite low by the lipofection method. Electroporation method will be examined. The condition are from Prof Takagi (ask Marina). 

Radiation experiments should be done after successful disruption of the ATM gene in EdomiPS#S31 cells.

Ataxia Telangiectasia SNP analysis

http://blogs.yahoo.co.jp/akihiroumezawa/34178477.html

The SNP genotyping by SNP array data was uploaded to the ncbi web site (GSE47498: Increased X-ray sensitivity and sustained chromosomal stability in Ataxia Telangiectasia-derived induced pluripotent stem (AT-iPS) cells, GSM1151202: AT1OS cells, GSM1151203: ATiPS-262 cells at passage 17, GSM1151204: ATiPS-263 cells at passage 27, GSM1151205: ATiPS-264 cells at passage 25, GSM1151206: ATiPS-024 cells at passage 25).