Saeko method.

1. 1 x 10^4 cells per well in a 96-well dish are cultivated for EB formation

2. EB formation in the EB medium for 10 days

EB medium

Knockout DMEM (380 ml)

KSR (100 ml)

Pc/SM (5 ml)

GlutaMax (5 ml)

Sodium Pyruvate (5 ml)

MEM (5 ml)

beta-ME (0.5 ml)