Saeko with Kenta and Takaaki

Spontaneous differentiation

EB formation by the Saeko method.

Adhesion on collagen for seven days.

Tic was differentiated by the Saeko method with adhesion on collagen for seven days.

Test

A.  Morphology

B.  qRT-PCR 

C.  in vivo (not done)

D.  Paraffin-embedded slides

E.  Immunocytochemistry (fluorescent)

F. EM

Cell

A.MRCiPSC#51 (intermediate)---number of tubes: appropriately 5.

B.EdomiPSC#31 (most efficient hepatic differentiation)---number of tubes: 5.

C.MRCiPSC#16 (least efficient)---number of tubes: appropriately 5.

D.MRCiPSC#25(Tic) (intermediate) 

Repeating above experiments (July 13, 2015)