Takaaki

Hep2044iPS#4

Takaaki method

ALB and AFP expression ten times

1. BA-iPSCs

TY uses Yu's iPSCs, Hep2044 form hepatocytes of BA.

A. Four lines (#2 to #5) from Hep2044 (BA).

See Yu site.

Hepatic differentiation of BA-iPSCs.

A.  Saeko method.

B.  HAES SOP.

pHAES-WCB (Starting material) -> pHAES10 -> EB96 -> HAES a.Propagation with MEF in KSR. b.Period of EB formation i.4 days in XF(-) (= EB medium) in 96-well dishes. c.Period of adhesion. i.28 to 35 days in XF32.

C.  Low molecules published in Stem Cell Reports.

D.Takaaki method (Saeko-modified method)

a.Propagation with MEF in iPSellon.

b.Period of EB formation

i.10 days in XF32 in 96-well dishes.

c.Period of adhesion.

i.  7 days in XF32. 

Test

A.  Morphology

B.  qRT-PCR

C.  in vivo

D.  Paraffin-embedded slides

Exome analysis and karyotypic analysis of BA-iPSCs will be performed. 

2. Hep2013 BA-iPSCs (A) and FH-iPSCs (B).

A.CXY-37 BA-iPSC: Nine lines from Hep2013 (BA) with Xiao.

Ike-59 BA-iPSC by Yu.

B. Kenta's Hep(C) FH-iPS#62 and #66.

Test as iPSC undifferentiated state.

Exome analysis and karyotypic analysis of BA-iPSCs will be performed.